Journal: Cell and Tissue Research

Article Title: Acute myocardial infarction does not affect functional characteristics of adipose-derived stem cells in rats, but reduces the number of stem cells in adipose tissue

doi: 10.1007/s00441-015-2239-z

Figure Lengend Snippet: Effect of AMI on rat SVF cells. a Cell size (μm) of the SVF cells, demonstrating no difference between the three groups. b Percentage of colony-forming cells, showing significantly fewer colonies in the 1D group (6.1 ± 1.6 %) compared to the Control (11.4 ± 1.8 %) and 7D groups (11.0 ± 0.9 %). c Number of cells, obtained 4 days after seeding of the SVF fraction, demonstrating significantly fewer cells in the 1D group (1.3 ± 0.1 million cells) compared to the Control (2.0 ± 0.1 million cells) and 7D groups (1.8 ± 0.1 million cells). d Percentage of positive cells for the markers CD31, CD34, CD45, CD73, CD90, CD105 and CD271 showing significantly fewer CD90- and CD105-positive cells in the 1D group. Data shown as mean ± SD (Control and 7D group n = 6, 1D group n = 5, * p < 0.05, ** p < 0.01)

Article Snippet: The following primary antibodies were used: phycoerythrin (PE)-conjugated mouse-α-rat antibodies against CD90 (1:20; Cedarlane Laboratories, Ontario, Canada), CD45 (1:20; BD Biosciences, San José, CA, USA), CD271 (1:25; BD Biosciences) and CD31 (1:20; AbD Serotec, Oxford, UK), PE-labeled mouse α-human antibody CD105 (1:25; Caltag, Invitrogen, USA), as well as unlabeled mouse-α-rat antibodies against CD34 (1:20; Santa Cruz Biotechnology) and CD73 (1:20; BD Biosciences).

Techniques: Control

Journal: Cell and Tissue Research

Article Title: Acute myocardial infarction does not affect functional characteristics of adipose-derived stem cells in rats, but reduces the number of stem cells in adipose tissue

doi: 10.1007/s00441-015-2239-z

Figure Lengend Snippet: Effect of AMI on cultured rat ASC. a Representative light microscopy image demonstrating the morphology of cultured rat ASC. b Cell size (μm) of ASC, demonstrating no significant differences between the three groups. c Population doubling time (in days) depicted for passages 1–3, showing no significant differences between the three groups. d Percentage of positive cells for the markers CD31, CD34, CD45, CD73, CD90, CD105 and CD271, showing no significant differences in numbers of positive cells between the groups. Data shown as mean ± SD (Control and 7D group n = 6, 1D group n = 5)

Article Snippet: The following primary antibodies were used: phycoerythrin (PE)-conjugated mouse-α-rat antibodies against CD90 (1:20; Cedarlane Laboratories, Ontario, Canada), CD45 (1:20; BD Biosciences, San José, CA, USA), CD271 (1:25; BD Biosciences) and CD31 (1:20; AbD Serotec, Oxford, UK), PE-labeled mouse α-human antibody CD105 (1:25; Caltag, Invitrogen, USA), as well as unlabeled mouse-α-rat antibodies against CD34 (1:20; Santa Cruz Biotechnology) and CD73 (1:20; BD Biosciences).

Techniques: Cell Culture, Light Microscopy, Control

Journal: DNA and Cell Biology

Article Title: Fibroblast Growth Factor 1-Transfected Adipose-Derived Mesenchymal Stem Cells Promote Angiogenic Proliferation

doi: 10.1089/dna.2016.3546

Figure Lengend Snippet: Flow cytometry characterization of AD-MSCs. MSCs expressed mesenchymal markers CD29 and CD90 (left) and were negative for hematopoietic markers, CD34 and CD45 (right). AD-MSCs, adipose-derived mesenchymal stem cells.

Article Snippet: The cells were incubated for 20 min at 4°C in the dark room with the following antibodies: CD90-phycoerythrin (PE) (NB100-65543PE; Novus Biologicals), CD29-fluorescein isothiocyanate (FITC, BD-561796; BD Biosciences, Franklin Lakes, NJ), CD45-FITC (bs-4819R; Bioss), and CD34b-PE (ABIN671373; Antibodies-Online).

Techniques: Flow Cytometry, Derivative Assay